Hemostasis,thrombosis, and Vascular Biology

Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the persistent presence of antiphospholipid antibodies (aPLs) and recurrent thrombosis or fetal loss. The thrombophilic state has been partially related to the induction of a proinflammatory and procoagulant endothelial cell (EC) phenotype induced by anti– 2-glycoprotein I ( 2-GPI) antibodies that bind 2-GPI expressed on the EC surface. Anti– 2-GPI antibody binding has been shown to induce nuclear factorB (NFB) translocation leading to a proinflammatory EC phenotype similar to that elicited by interaction with microbial products (lipopolysaccharide [LPS]) and proinflammatory cytokines (interleukin 1 [IL1 ], tumor necrosis factor [TNF]). However, the upstream signaling events are not characterized yet. To investigate the endothelial signaling cascade activated by anti– 2-GPI antibodies, we transiently cotransfected immortalized human microvascular endothelial cells (HMEC-1) with dominant-negative constructs of different components of the pathway ( TRAF2, TRAF6, MyD88) together with reporter genes (NFB luciferase and pCMV-galactosidase). Results showed that both human anti– 2GPI IgM monoclonal antibodies as well as polyclonal affinity-purified anti– 2-GPI IgG display a signaling cascade comparable to that activated by LPS or IL-1. TRAF6 and MyD88 significantly abrogate antibody-induced as well as IL-1– or LPSinduced NFB activation, whereas TRAF2 (involved in NFB activation by TNF) does not affect it. Moreover, anti– 2-GPI antibodies and LPS followed the same time kinetic of IL-1 receptor–activated kinase (IRAK) phosphorylation, suggesting an involvement of the toll-like receptor (TLR) family. Our findings demonstrate that anti– 2-GPI antibodies react with their antigen likely associated to a member of the TLR/IL-1 receptor family on the EC surface and directly induce activation. (Blood. 2003;101:3495-3500)